λ DNA digested with Fnu4HI (SatI), 1.4% agarose, 380 cleavage sites

FastDigest enzymes are a high-end line of fast restriction enzymes that are 100% active in both Universal FastDigest Buffer and FastDigest Green Reaction Buffer.

The universal buffer can quickly complete DNA single, double or multiple enzyme digestion in 5-15 minutes without changing the buffer or subsequent DNA purification steps. DNA modifying enzymes such as Klenow fragment, T4 DNA ligase, alkaline phosphatase, and T4 DNA polymerase are 100% active in FastDigest buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture.

For convenience, FastDigest Green Buffer contains a density reagent and two tracking dyes for direct gel loading of PCR products.

Universal FastDigest buffer eliminates the star activity effect due to its short incubation time and optimal combination of ingredients.