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Amplified from human genomic DNA over a wide range of Mg2+ concentrations A single-copy gene of 950 bp was produced.
M - GeneRuler 100 bp Plus DNA Molecular Weight Standard
Taq DNA polymerase is a highly thermostable DNA polymerase derived from thermophilic bacteria Thermus aquaticus. This polymerase catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity, and has low levels of 5'→3' exonuclease activity. In addition, Taq DNA polymerase also has deoxynucleotidyl transferase activity, which often results in the addition of additional adenine at the 3'-end of the PCR product. RecombinantTaqDNA polymerase is ideal for standard PCR with templates of 5 kb or less.
PCR Master Mix is a 2X concentrate containing Taq DNA polymerase, dNTPs, and all other components required for PCR, excluding DNA template and primers. This premixed formulation saves time and reduces contamination by reducing the number of pipetting steps required to prepare a PCR reaction. Mixed solutions are optimized for efficient and reproducible PCR.