The Thermo Scientific TranscriptAid T7 High-Yield Transcription Kit contains reagents for 50 reactions (20 μL each). Each reaction generates up to 200 µg of RNA from 1 µg of template in 2 hours. Reactions can be scaled up to produce milligram quantities of full-length RNA. This kit can be used to generate both long and short transcripts for applications requiring higher RNA yields. Includes all reagents necessary for transcription, plus RiboRuler High Range RNA Typing Standard.

Product Advantages

•Extremely high yields—up to 200 μg in 2 hours
• Universal —Suitable for short and long RNA transcripts
• Milligram-scale RNA in single, scale-up reactions
• Flexible— Generates unlabeled, labeled or capped RNA
• RiboRuler RNA Typing Standards are provided with the kit for size analysis and quantitation

applications

• In vitroTranscription
• In vitroTranslation
• Generate hybridization probes for:
• Micro Array
• In situ Hybridization
• Blotting

• RNase Protection Assay
• RNA Binding Protein Assay
• Antisense RNA and RNAi
• RNA Amplification
• Microinjection Studies

Includes

• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
• ATP solution
• CTP solution
• GTP solution
• UTP solution
• Control template
• 3 M sodium acetate, pH 5.2
• DEPC-treated water
• 2X RNA loading dye
• RiboRuler High Range RNA Typing Standard, Ready to Use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol

* TranscriptAid T7 An improved version of the High Yield Transcription Kit contains a Tris-buffered NTP solution instead of the previously used NTP solution titrated with KOH. Using Tris-buffered NTP solutions results in higher yields of RNA transcripts.