Thermo Fisher FD0664 200 reactions FastDigest SmaI
$93.00 / Parcel$110.74 Min.order:1
Brand Sales
FD0664 200 reactionsFastDigest SmaI

Support Payment Term:

HSBC Hong Kong

paypal

Alibaba Pay

Western Union

USD

EUR

GBP

SGD

HKD

CNH

CAD

MXN

BRL

JPY

THB

MOP

AUD

NZD

PLN

CZK

HUF

RON

CHF

SEK

NOK

DKK

TRY

AED

SAR

ILS

ZAR

$0.00

Quantity

Discover similar items

Page 1 of 2

5' C C C ↓ G G G 3'
3' G G G ↑ C C C 5'


Thermo Scientific FastDigest SmaI restriction enzyme recognizes the CCC^GGG site and cleaves well within 5–15 minutes at 37°C using universal FastDigest buffer. Iso-cleavage enzymes: TspMI, XmaCI, XmaI.

Thermo Scientific FastDigest SmaI is a premium line of fast restriction enzymes that are 100% active in both universal FastDigest and FastDigest Green reaction buffers.

Universal buffer allows rapid single, double or multiple enzyme digestion of DNA in 5–15 minutes without the need for buffer changes or subsequent DNA purification steps. See FastDigest Enzyme Reaction Conditions table for enzymatic activity, heat inactivation, and incubation of this and other FastDigest restriction enzymes time. DNA modifying enzymes (such as Klenow fragment, T4 DNA Ligase, alkaline phosphatase and T4 DNA Polymerase) are 100% active in FastDigest buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture.

For convenience, FastDigest Green buffer includes 1 density reagent and 2 tracking dyes for loading digestion reaction products directly onto the gel.

Shorter incubation times and optimized universal FastDigest buffer composition eliminate star activity effects.

Features

• All FastDigest enzymes are 100% active in universal buffers
• 100% buffer compatible with downstream applications< br>• Complete digestion in 5–15 minutes
• Load directly onto gel
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blot
• Restriction fragment length polymorphism (RFLP)< br>• SNP Analysis

Note: FastDigest Green Buffer has the same high performance as colorless FastDigest Buffer in DNA digestion reactions and downstream applications. For applications requiring product analysis by fluorescence excitation (eg determination of concentration under UV light), the use of colorless FastDigest buffer is recommended.

See product specifications for information on methylation sensitivity.
For Research Use Only. Not for use in diagnostic procedures.