Thermo Scientific GeneJET PCR Purification Kit uses proprietary silica membrane technology in a convenient spin column format, eliminating the need for expensive resins or toxic Phenol-chloroform extraction. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes and salts from PCR and other reaction mixtures.
This kit can be used to purify DNA fragments from 25 bp to 20 kb with a recovery rate of up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg DNA, and the entire process only takes 5 minutes. The purified DNA can be used for common downstream applications, including sequencing, restriction enzyme digestion, labeling, ligation, cloning,
in vitro transcription, blotting or
in situ hybridization, etc.
Product Advantages•
Efficient: In the range of 100 bp to 10 kb, the recovery rate is 90% to 100% < br>•
Fast: The procedure takes only 5 minutes
•
Convenient: The spin column is capped and equipped with a collection tube
Application b>
•Fast and efficient purification of DNA fragments, ideal for all routine molecular biology procedures, including:
•
FastDigest or
Conventional restrictiondigestion
•Automated fluorescent or radioactive sequencing
• PCR
•
In vitrotranscription
•
In situhybridization
•Marking
•Cloning
For Research Use Only. Not for use in diagnostic procedures.