Thermo Scientific GeneJET Whole Blood RNA Purification Miniprep Kit is designed for the rapid and efficient purification of RNA from whole blood and related body fluids. Quality of total RNA.

The kit uses silica membrane technology in a convenient spin column format, eliminating the need for expensive resins, toxic phenol-chloroform extraction, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. High-quality purified RNA can be used in a variety of downstream applications such as RT-PCR, RT-qPCR, Northern blotting, and other RNA analyses.

Product Advantages

•Efficient – Obtain up to 1.8 µg of total RNA from 500 µL of healthy human blood
•< b>Convenience – Spin columns are capped and equipped with collection tubes
•High Purity – RNA is free of enzyme inhibitors, proteins and ready for use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCR
•Fast and simple – Procedure takes less than 15 minutes and does not involve organic extraction or sorting RBC lysis
•Compatible – Works with a wide range of samples – human and animal blood, buffy coat, bone marrow and related body fluids treated with EDTA, citrate or heparin

Applications

• Rapidly extract high-purity RNA suitable for all routine molecular biology procedures, including:
•RT-PCR
• RT-qPCR
• Northern blot
• Nucleic acids Enzyme protection test includes lysis buffer, wash buffer WB 1 (concentrate), wash buffer 2 (concentrate), nuclease-free water, GeneJET RNA purification column (pre-installed collection tube), collection tube (2 mL) , collection tube (1.5 mL), detailed protocol