Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit uses silica membrane technology in a convenient spin column format, eliminating the need for expensive resin, toxic phenol-chloroform extraction or time-consuming alcohol precipitation. Standard procedures after cell lysis take less than 20 minutes and yield purified DNA greater than 30 kb in size. The isolated DNA can be used directly for PCR, Southern blotting and enzymatic reactions.

Product Advantages

•High Efficiency—high yield of high molecular weight genomic DNA
•Fast—The post-cell lysis procedure only takes 20 minutes
•Convenient—The spin column is capped and equipped with a collection tube
•High Purity—Pure DNA, Free of enzyme inhibitors, RNA and proteins. Ready for all demanding applications and validated in real-time PCR, end-point PCR, multiplex PCR and restriction enzyme digests
•Compatible—for A variety of samples such as fresh or frozen anticoagulated mammalian blood (treated with EDTA, citrate or heparin), time-stored blood, buffy coat, bone marrow and body fluids

Applications

• Isolated genomic DNA is ideal for common molecular biology procedures, including:
•PCR and qPCR
• FastDigest and conventional restriction enzyme digestion
•Southern blotting Includes proteinase K solution, lysis buffer, wash buffer WB I (concentrated), wash buffer II (concentrated), elution buffer, GeneJET purification column (pre-assembled with collection tube), collection tube, detailed protocol