RiboMinus Concentration Module is used to concentrate RiboMinus RNA while retaining materials less than 200 nucleotides (nt) for further for downstream applications.

Typically, RNA species smaller than 200 nt are removed from silica spin columns under standard binding conditions for RNA isolation. Research has demonstrated the importance of these small RNA species, which include regulatory RNA molecules such as microRNA (miRNA), short interfering RNA (siRNA), and snRNA. To retain RNA <200 nt in the concentration step of RiboMinus RNA isolation, the RiboMinus Concentration Module is used with a modified silica spin column protocol (using 60% ethanol for RNA binding).

The spin column protocol binding conditions are optimized for RiboMinus RNA samples using ethanol and buffer. Load the sample onto the spin column. RiboMinus RNA is bound to the silica membrane in the column and impurities are removed by thorough washing with wash buffer. RiboMinus RNA is then eluted into RNase-free water.