PureLink™ HiPure Plasmid Midiprep Kit is designed to isolate transfection grade from E. coli Plasmid DNA. The Midiprep Kit protocol typically yields 100–350 µg of plasmid DNA from 15–25 mL of bacterial culture with a purity comparable to that obtained by two passes through a cesium chloride gradient.

Advantages of the PureLink™ HiPure Plasmid Midiprep Kit include:

•Effort-saving and time-saving — no additional steps are required to remove the Toxins and other contaminants
• High purity, low endotoxin product — pure enough to transfect mammalian cells
• Versatility — can purify any type and sized plasmid DNA, including BAC, bacmid, and ssM13 DNA

Anion Exchange Chromatography for Plasmid Purification
PureLink™ HiPure Plasmid DNA Purification Kits use patented Anion exchange resin purifies plasmid DNA to a level equivalent to two passes through a CsCl gradient. The resin combines exceptional capacity with fast flow rates, high resolution, high yields and efficient endotoxin removal. Plasmid preparation is typically completed within 2 hours.

Contaminant-free DNA
Unlike cesium chloride gradient protocols, the PureLink™ HiPure Plasmid Purification System does not use organic solvents, ethidium bromide or cesium chloride, all of which Reagents are troublesome to operate and handle. Generally, the A₂₆₀/A₂₈₀ ratio of plasmid DNA prepared using the PureLink™ HiPure Plasmid Midiprep Kit is greater than 1.80, indicating that the DNA does not reasonably contain potential for downstream interference. Applied protein. Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for transfection of mammalian cells