The PureLink HQ 96 Plasmid DNA Purification Kit is designed for high-throughput isolation of high-quality plasmid DNA from bacterial cells. It can be used with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.

The PureLink HQ 96 Plasmid DNA Purification Kit offers the following advantages:
• Ability to isolate up to 10 μg plasmid DNA per well
>• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handling workstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfection

Process overview
Bacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in the plate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.