Features of these indicators include:
• Susceptibly oxidized by superoxide, but not other ROS or RNS generating systems
• For use in live cell imaging
• Rapidly and selectively targets mitochondria< br>• MitoSOX Green indicator: upper absorption/emission wavelength ∼488/510 nm (traditional FITC/GFP filter set)
• MitoSox Red indicator: upper absorption/emission wavelength ∼396/610 nm&ast; (Custom filter set recommended)

&ast;Robinson et al. (1) reported that the MitoSOX Red dye may be a more efficient source of mitochondrial superoxide if it is excited at 396 nm instead of 510 nm. Selective markers.

MitoSOX Green indicators are supplied as one vial or in a pack of five vials. MitoSOX Red indicators are available as one vial or in a pack of ten vials. We also offer mixed packs of one each of MitoSOX Red and MitSOX Green indicators.

Quick and simple detection of mitochondrial superoxide in living cells
MitoSOX Superoxide Indicator can be used to visualize mitochondrial superoxide production by fluorescence microscopy. Indicators penetrate living cells and selectively target mitochondria therein. These agents are rapidly oxidized by superoxide but not by other reactive oxygen species (ROS) and reactive nitrogen species (RNS). Oxidation products emit strong fluorescence.

MitoSOX Indicator can be used to distinguish artifacts from isolated mitochondrial preparations from direct measurements of superoxide generated in mitochondria of living cells. They can also be valuable tools when studying factors that regulate oxidative stress in various pathologies. Additionally, these indicators are used in metabolic flux determinations using high-content instrumentation (2). MitoSOX indicator has also been used to detect mitochondrial superoxide using flow cytometric analysis (3).

References
: 1: Robinson, Kristine M., et al.Selective fluorescent imaging of superoxide in vivo using ethidium-based probes.Proceedings of the National Academy of Sciences 103.41 (2006): 15038-15043.
2: Little, Andrew Charles, et al. High-content fluorescence imaging with the metabolic flux assay reveals insights into mitochondrial properties and functions. Communications biology 3.1 (2020): 1-10.
3: Kauffman, Megan E. et al.MitoSOX-Based Flow Cytometry for Detecting Mitochondrial ROS.Reactive oxygen species (Apex, NC) vol. 2,5 (2016): 361- 370.