Newport Green™ DCF indicator has moderate zinc binding affinity (K for Zn²⁺ _d ∼1 µM) but basically insensitive to Ca²⁺ (Kd for Ca²⁺ >100 µM), making it Becoming a valuable probe for detecting Zn²⁺ entering neurons through voltage-gated channels or glutamate-gated channels. Newport Green™ DCF confirms when used with dyes with dual Ca²⁺/Zn²⁺ sensitivity such as fura-2 and mag-fura-2 Changes in Zn²⁺ levels could be detected, but no changes in Ca²⁺ or Mg²⁺ levels could be detected. The response of Newport Green™ DCF to transition metals including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd was characterized.

Learn more about ion indicators, including calcium, potassium, pH, and membrane potential indicators›

Newport Green™ PDX has better performance than Newport Green™ DCF Indicator High Zn²⁺ dissociation constant (K_d for Zn²⁺ is ∼30 µM) and large Zn ^{The fluorescence intensity increases from 2+}-free to Zn²⁺- saturated.

Fluorescent zinc indicator specifications:
• Label (excitation/emission wavelength): Newport Green™ DCF (∼505/535 nm)
• K_d (Zn²⁺) (in buffer): ∼1 µM
• Lyophilized products can be dissolved in DMSO for use
• Typically, by adding to cell-containing cultures Add a dissolution indicator to the base and load the product into cells

Find fluorescent indicators for zinc and other metal ions
We offer a variety of fluorescent indicators to determine intracellular concentrations of multivalent cations, track metal ion transport through ion channels, perform measurements on environmental samples, and more. For more information about these products, please view Fluorescent indicators for Zn²⁺ and other metal ions—Molecular Section 19.7 in the Probes™ Manual.

For scientific research use only. Not for therapeutic or diagnostic use in humans or animals.