AmpErase™ Uracil N-Glycosylase (UNG) is a component of the GeneAmp™ PCR Carry-over Prevention Kit. It is a 26 kDa ultrapure recombinant enzyme encoded by the uracil N-glycosylase gene in Escherichia coli. It is inserted into the Escherichia coli host to directly express the wild type Enzymes. This enzyme removes any uracil incorporated into single- or double-stranded DNA.

GeneAmp™ PCR Carry-over Prevention Kit
The reagents provided in the GeneAmp™ PCR Carry-over Prevention Kit (sold separately) enable a simple yet powerful This method ensures that the PCR product will not be amplified again in subsequent PCR amplification, thereby preventing false positive results. Features of this kit include:

• Enzymatic method prevents contamination by PCR residues, thereby avoiding false positive results
• Can degrade the PCR product amplified by upstream PCR without degrading the natural nucleic acid template, thus Improved amplification quality
• Will not interfere with any PCR or real-time PCR applications
• Optimized for use with GeneAmp™ PCR Core Reagents and GeneAmp™ Instrument Systems

Clean PCR for good Residual contamination
This kit uses an enzymatic reaction similar to the restriction modification and excision repair system of cells to specifically degrade the PCR product incorporated with dUTP in the upstream PCR amplification without degrading the natural Nucleic acid template. Methods to render PCR products susceptible to degradation require replacing dTTP in the PCR mixture with dUTP and pretreating all subsequent PCR mixtures with AmpErase™ Uracil N-Glycosylase (UNG) prior to PCR amplification. PCR amplification products contain uracil and can be easily distinguished from DNA templates that naturally contain thymidine. Removal of uracil residues by UNG and thermal degradation of the resulting abasic polynucleotide can completely eliminate the products of upstream PCR amplification. Although UNG is active on both single and double strands containing dU, the ribouracil residues of RNA and dUTP themselves are not substrates of UNG. AmpliTaq™ DNA Polymerase, AmpliTaq Gold™ DNA Polymerase, and other components of the PCR mix remain fully active during UNG treatment, allowing PCR amplification without carryover of PCR product. dU-containing PCR products are handled similarly to dT-containing DNA in blotting, cloning, sequencing, and most other downstream analyses.