NuPAGE LDS Loading Buffer (4X) for denaturing gels using Bis-Tri or Tris-acetate gels Preparation of protein samples for electrophoresis applications. It contains lithium dodecyl sulfonate (pH 8.4) to maximize reducing agent activity.

View all buffers and reagents available for SDS-PAGE

The tracer dyes contained in the NuPAGE LDS loading buffer are Coomassie G250 and phenol red, not bromophenol blue. Coomassie G250 shows a clear dye front in both MES and MOPS SDS running buffers and is closer to the moving ion migration front than bromophenol blue. When using MES SDS electrophoresis buffer, bromophenol blue migrates slower than some peptides. This ensures that small-sized peptides do not migrate on the gel.

Instructions: Heat 1X diluted samples (reduced or unreduced) at 70°C for 10 minutes for ideal results.

Note: NuPAGE LDS loading buffer should be brought to room temperature (25°C) before use. It is a low-viscosity, concentrated solution that contains twice the LDS content of commonly used sample buffers. NuPAGE LDS sample buffer also contains a higher concentration of glycerol.