Labeled calcium indicators are molecules that show an increase in fluorescence upon binding to Ca²⁺. They can be used for a variety of calcium signaling studies, including measurement of Ca²⁺ in cells and tissues with high levels of autofluorescence and Ca²⁺ produced by photoreceptors and photoactivated chelators. sup> release detection. The AM ester form of this calcium indicator can be loaded onto the cells by adding the dissolved indicator directly to the culture dish containing the cultured cells. Fluorescence signals from these cells are typically detected using fluorescence microscopy.

Calcium Indicator (AM Ester) Specifications:
• Label (Excitation/Emission Wavelength of Ca²⁺– bound form): Rhod-2 (552/ 581 nm)
• Fluorescence intensity increases after binding Ca²⁺: >100 times
•Ca ^{when there is no Mg2+ in the buffer K_d of 2+}: ∼570 nM
• After binding to Ca²⁺, the fluorescence increases and the wavelength changes slightly

Control of heavy metal cations using TPEN
In addition, BAPTA-based indicators can bind various heavy metal cations (such as Mn²⁺, Zn^{2 +}, Pb⁺²), the affinity is much higher than Ca²⁺. Perturbations in calcium measurements due to the presence of these ions can be controlled using the heavy metal selective chelator TPEN.

More Choices of Fluorescent Calcium Indicators
We offer a large selection of Molecular Probes™ calcium indicators that can be used in a variety of experimental protocols, such as dextran forms to reduce leakage and zone Compartmentalized, BAPTA conjugates are used to detect high-amplitude calcium transients. For more information, check out the Molecular Probes™ Handbook Fluorescent Ca²⁺ indicators excited with visible light - Chapter Section 19.3.

For UV excited Ca²⁺ indicators, protein-based Ca²⁺ indicators, Ca²⁺ indicators Conjugates of reagents and fluorescent indicators of other metal ions (i.e. Mg²⁺, Zn²⁺), please see Ca²⁺, Mg²⁺, Zn²⁺, and other metal ion indicators—Chapter 19.

For scientific research use only. Not for therapeutic or diagnostic use in humans or animals.