Exosome-streptavidin isolation/detection reagent combined with your choice of biotinylated primary antibody can Purification of exosomes (also known as extracellular membrane vesicles and multivesicular bodies) from pre-enriched samples. These exosomes can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. Exosomes must be pre-enriched before isolation. This can be achieved by ultracentrifugation or using reagents for rapid and efficient isolation of total exosomes (from cell culture media).

• Greater flexibility in your choice of biotinylated antibodies
• “See” your samples during processing
• Easily scalable protocols
• In Detection of exosomes by flow cytometry in less than 1 hour

Isolating and detecting exosomes is a time-consuming, tedious, non-specific and difficult process. Exosome-streptavidin isolation/detection uses the well-known Dynabeads™ magnetic separation technology. When combined with a biotinylated antibody of your choice, this technology allows you to easily purify pre-enriched exosomes from cell culture media and then further detect the purified exosomes by techniques such as flow cytometry, electron microscopy, or Western blotting. exosomes.

Detection by Flow Cytometry
One of the main advantages of using magnetic separation technology is that individual free exosomes are too small to be detected by flow cytometry. Purified bead-bound exosomes can be easily visualized by flow cytometry. Monodisperse and relatively large Dynabeads™ (4.5 µm diameter) typically provide clear and unambiguous FFC/SSC in less than 1 hour.

Can "see" your sample
Superparamagnetic Dynabeads™ not only have known sensitivity, reproducibility and stability, but also have a light brown color due to the microbeads , and can “see” your sample during magnetic processing. When the sample tube is placed on the magnetic stand, the exosomes bound to the magnetic beads are pulled to the side of the tube, allowing for easier isolation and purification. Additionally, sample and bead volumes can be easily scaled up or down based on sample volume or downstream application.

Good mixing is critical
For successful exosome isolation, be sure to use a mixer with a tilt and rotate mixer to ensure that the beads do not settle in the in the tube. Avoid end rotation for small sample volumes (e.g., 100 µl). See the user manual below for more guidance.