Use this kit to isolate pure and viable unexposed NK cells from PBMC by negative isolation. This kit removes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and red blood cells. Negatively isolated human NK cells remain in the sample and are not in contact with Dynabeads. Microbeads and antibody-free NK cells are truly contact-free and appear to be in perfect shape for any functional assay and application.

• High purity, recovery and survival of human unexposed NK cells
• Easy to use and amplify
• No columns required

Method:< /b>
An anti-non-NK cell antibody cocktail is added to the sample and allowed to bind to the cells. Dynabeads™ are added and bind to antibody-labeled cells during a short incubation period. Bead-bound cells on the magnetic rack are rapidly detached and discarded. The remaining negatively isolated and unexposed human NK cells can be analyzed directly in flow cytometry and used in any application.

Starting sample
: PBMC