This gentle and easy-to-use kit isolates highly pure and functional mouse Treg cells from mouse lymph node or spleen samples . Your cells are not exposed to pressure conducted through the column. Isolated CD4+CD25+Foxp3+ Treg cells are magnetic bead-free and compatible with flow cytometry. Cells can be expanded very easily and retain their functional inhibitory capabilities. It is expected to obtain >90% purity grade Treg cells. More than 88% of isolated CD25+ cells express Foxp3. You'll save time and money and gain greater confidence in your results.

Method:
Negatively isolate CD4+ T cells, and then positively isolate CD25+ regulatory T cells. First, a cocktail of antibodies is added to the sample, and the antibodies will bind to non-CD4+ T cells. Mouse Depletion Dynabeads™ are then added, which will bind to the antibody-cell complexes within a short incubation time. Cells bound to magnetic beads were detached on a magnetic stand and discarded. The remaining unexposed mouse CD4+ T cells were then labeled with FlowComp™ CD25 Antibody and positive isolation was performed using FlowComp™ Dynabeads™. Isolated mouse regulatory T cells are released from Dynabeads™ after a short incubation with FlowComp™ Release Buffer. This isolation method is easy to use and scalable.

Advantages:

CD4+CD25+Foxp3+ Treg cells are of high purity.

• No risk of results being affected by the separation method
• Gentle tube-based method - no columns required
• High purity, recovery and viability
• CD25+Foxp3+ High Express.
• Bead-free cells - compatible with flow cytometry analysis
• Treg cells can be expanded very easily and retain their functional suppressive capabilities.

Starting sample:
Spleen or lymph node cells