Dynabeads™ M-450 epoxy beads are ideal for custom cell isolation solutions upstream of molecular applications. Purified antibodies can be combined with these microspheres to produce magnetic cell separation media with good antibody orientation

This product is ideal for cell separation when using custom antibodies:
Because the background is low and the antibodies are covalently attached to the bead surface, these beads are ideal for cell separation applications when downstream release of beads is not required.

Ultra-fast solutions:

Rapid isolation of target cell populations upstream of molecular analysis

• Ideal for cell isolation processes requiring custom antibodies
• Couple different custom antibodies to different beads to screen for specific cell subsets

Not recommended for immunoprecipitation (IP):
Not recommended These 4.5 micron beads were used for IP because they have a lower surface capacity per unit mass than the epoxy-coated 1 µm and 2.8 µm p-toluenesulfonyl Dynabeads™. Use Dynabeads Protein A (10001D and 10002D), Dynabeads Protein G (10003D and 10004D), or one of the Dynabeads Immunoprecipitation Kits (10006D and 10007D)

Overview of the coupling procedure:< br>Any purified antibody can be covalently coupled to the bead surface via an easy-to-perform overnight reaction. Simply incubate the antibodies to be used in the cell isolation process with these 4.5 µm Dynabeads™. Optimal coupling occurs at high pH (8.5-9.5) and 37°C. For pH-labile antibodies, coupling can be performed in other buffers at pH 7.4.

About Dynabeads™:
Dynabeads™ are nonporous monodisperse superparamagnetic microbeads. They are highly mobile in solution, allowing antibodies coupled to the beads to continuously interact with the cell suspension. When placed in a magnetic field, these 4.5 µm superparamagnetic beads experience strong attraction; when the test tube is transferred to a tube rack containing a strong magnetic field, the beads gently draw target cells to the tube wall. The beads and attached cells are concentrated on the tube wall, so the supernatant containing non-target cells can be poured off or aspirated easily and quickly. The washing steps are similar.

Dynabeads™ M-450 Epoxy Resin Microbeads Characteristics:
• Coated with glycidyl ether (epoxy resin) groups
• Hydrophilic, medium pH Properties
• Covalent coupling via primary amine (NH2) or sulfhydryl (SH) groups
• Large 4.5 µm bead size ideal for cell separation
• For IP applications, 4.5 µm is not recommended of large microbeads