Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with a highly sensitive Assay for detecting the transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysates.

GaussiaLuciferase Flash Detection Kit Features:

•Sensitive — 1000x increased sensitivity allows the use of less Cells
• Cost-Effective — Extremely sensitive assay reduces reagent consumption
• Secreted Luciferase — Enables real-time assays without damaging cells and kinetic studies
• Saving time — Assays with secreted luciferase require minimal sample handling
• Suitable for automation — Suitable for high-temperature applications Throughput Screening
• Convenient — Contains a universal cell lysis buffer and optimized flash assay reagents
• Safety — Allows users to perform non- Radioactivity Assay

This flash assay kit contains reagents for the determination of Gaussia luciferase activity in mammalian cell culture media and lysates. When used with the Thermo Scientific Gaussia Luc vector, this kit provides a very sensitive bioluminescent reporter assay system for secretory or intracellular detection of promoter or pathway activity. Gaussia Luciferase is highly secreted in cell culture media and enables live cell monitoring of reporter gene activity. Gaussia produced a much higher signal than that of firefly or Renilla luciferase assayed under similar conditions.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Gaussia Luciferin Enzymes and photometers or other instruments capable of monitoring luminescence (such as Thermo Scientific Luminoskan Ascent and Varioskan microplate readers).

Applications:
• Promoter studies for analysis of cis regulatory elements and trans acting factors< br>• Drug Screening
• siRNA and miRNA Screening
• Multi-Pathway Assays for Studying Off-Target Effects
• Secretory Pathway/Protein Localization Reporter Gene Assays
• Signal Transduction Pathway Analysis
• RNA splicing studies

Gaussia Luciferase is a 20kDa protein from the marine copepod Gaussia princeps. Bioluminescent enzymes are highly secreted in cell culture media and enable live cell monitoring of reporter gene activity. The light output produced by the luciferase reaction can be correlated with the amount ofGaussialuciferase protein produced and used to measure the activity of the promoter driving Gaussia expression. The signal generated by Gaussia luciferase exhibits strong flash kinetics and is higher than that of firefly or Renilla luciferase measured under similar conditions Much more. The assay is optimized for use with theGaussiaLuc vector but is fully compatible with otherGaussialuciferases and derivatives that use coelenterazine as a substrate. (For glow determination, use our Gaussia-Dura Luc vector.)

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