Thermo Scientific Halt Phosphatase Inhibitor Single Use Cocktail maintains protein phosphorylation during and after cell lysis or tissue protein extraction status.

Halt Phosphatase Inhibitor Single Use Cocktail Features:

•Lack of Protease Inhibitors—To protect proteins from proteases and phosphatases, Use Halt Protease and Phosphatase Inhibitor Cocktail.
•Dialysis—Samples can be dialyzed or desalted to effectively remove inhibitors from cell extracts before performing procedures incompatible with the components of the mixture.
•Available as a 100X concentrate—Also valid at a final concentration of 1X; however, samples containing particularly high levels of phosphatase activity may require a final concentration of 2-3X.
•Compatibility—Compatible with most detergent-containing cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Buffers.
•All-in-one format — Inhibits serine-threonine phosphatase and protein tyrosine phosphatase
•Compatible with commonly used protein assays — Available For use in standard protein assays, including BCA and Coomassie (Bradford) assays
•Convenient storage — Store in the refrigerator (2 to 8°C) to save valuable space in the freezer and Ready for immediate use

Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases and protein tyrosine phosphatase (PTP) during cell lysis. The cocktail contains a mixture of four broadly specific inhibitors, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerol phosphate. Covalently packaged in a 100x form, the mixture is stable at 4°C and suitable for use with any cell or tissue extract. The mixture allows proteins to remain phosphorylated during immunoprecipitation and kinase assays.

Phosphorylation and dephosphorylation include molecular switches that regulate many key biological pathways within cells, including signal transduction, cell division, and apoptosis. To effectively study the phosphorylation activity state, the user must inactivate the phosphatase to prevent its uncontrolled activity after cell lysis. Halt Phosphatase Inhibitor Cocktail does this.

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