NuPAGE antioxidants can prevent sample re-oxidation and maintain the reduced state of proteins during protein gel electrophoresis and transfer processes. When performing protein gel electrophoresis under reducing conditions, add it to the electrophoresis buffer. NuPAGE antioxidants migrate with reducing proteins to maintain the reduced state and prevent the reoxidation of sensitive amino acids such as methionine and tryptophan.

NuPAGE Antioxidants should be used with samples reduced with NuPAGE Sample Reducing Agent or other reducing agents With the use of. NuPAGE Antioxidants are optimized for the neutral pH system of NuPAGE gels and are not compatible with other gel chemistries. NuPAGE transfer buffer can also be added to enhance protein transfer to the membrane.

View all buffers and reagents available for SDS-PAGE