McCoy's 5A (modified) medium is a universal medium that supports many types of primary cells, established Proliferation of cell lines as well as explants of biopsy tissue. This medium supports the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryo, and other tissues.
Complete available Formulation.

Using McCoy's 5A (Modified) Medium
Thomas McCoy originally formulated McCoy's 5A Medium as a modified version of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacterial peptone, and high concentrations of glucose. This product also includes Dr. Hsu Hanks' salt additive to support use outside of a CO₂ incubator. McCoy's 5A (modified) medium requires the addition of serum, usually 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP Manufacturing and Quality Systems
McCoy's 5A (Modified) Medium is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same McCoy's 5A (modified) media product (26600-080) produced in our Scottish factory. The latter is also a medical device manufacturer registered with the FDA and complies with the ISO 13485 standard.