Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mix containing Phusion Plus DNA Polymerase, buffer, magnesium chloride and dNTPs. The master mix format simplifies reaction preparation with minimal pipetting steps and retains all the properties of Phusion Plus DNA Polymerase. In addition, two tracking dyes and density reagents have been added to this master mix to allow direct loading of PCR products onto the gel.

•High fidelity — Sequence accuracy >100 times higher than Taq polymerase
• Easy to set up — Use the same sequence in PCR Annealing temperature 60°C
• Higher specificity — Hot start mechanism reduces non-specific amplification and primer degradation
• Bench stability — Formulation A good reaction system is stable for 24 hours and can be automated
• High inhibitor tolerance — suitable for DNA with poor purity

Phusion Plus DNA Polymerase is a thermal Initiate high-fidelity DNA polymerase using protein fusion technology and universal primer annealing capabilities. It is a Pyrococcus -like proofreading DNA polymerase fused to a processivity-enhancing domain, allowing Phusion Plus Green PCR Master Mix to generate PCR sequences with high accuracy, sensitivity, and inhibitor tolerance. Its novel reaction buffer contains a proprietary additive that stabilizes the primer/template duplex during the 60°C annealing step. This unique buffer formulation improves PCR specificity by eliminating the need to optimize annealing temperatures for each primer pair individually, helping to save time and avoid human error in PCR runs. In addition, with Phusion Plus Green PCR Master Mix and its universal annealing function, different PCR reactions can be cycled simultaneously using the same protocol.

Phusion Plus Green PCR Master Mix is inactivated at room temperature due to the hot-start mechanism mediated by the parent molecule, allowing for preparation and storage of PCR reactions prepared at room temperature. Enzyme activity is not fully activated until the predenaturation step.

Applications
• High-fidelity PCR
• Cloning
• Generate sequencing templates
• Amplify complex (high GC content) templates
• Long fragment PCR (up to 20 kb)
• Mutation
• High-throughput PCR

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