Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that is different from traditional hot start Achieve higher PCR specificity, sensitivity and yield compared to Taq DNA polymerase.

DreamTaq Hot Start DNA Polymerase provides antibody-based inhibition of DNA polymerase activity at ambient temperature to prevent amplification of non-specific products prior to the amplification step. Equipped with DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocols and cycling conditions as traditional Taq DNA Polymerase. The enzyme is supplied with optimized 10X DreamTaq buffer containing magnesium chloride and does not require extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is available as a preloaded density reagent and electrophoresis tracking dye, as well as a master mix.

DreamTaq Hot Start DNA Polymerase uses the following formula to increase productivity:
•Better reaction results
--Higher target amplicon yields can be obtained from low template amounts< br> --Increased reaction specificity due to reliable hot-start technology
--Wider amplicon length range—routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
--Settings for reaction at room temperature
--Due to optimized reaction buffer, minimize Mg2+ concentration and primer annealing temperature optimization

Application:
•Conventional PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generate PCR products for TA cloning

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