Thermo Scientific Restore Western Blot Antibody Stripping Buffer safely and efficiently removes primary antibodies and PVDF membrane supernatants from nitrocellulose and PVDF membranes. Secondary antibodies were used to detect chemiluminescent Western blots again.

estore Western Blot Antibody Stripping Buffer Features:

• Save time—no need to re-make gels for electrophoresis
• Save precious samples—use the same sample on the same membrane and re- Detection
•High efficiency - patented formula, the removal effect is much better than homemade buffer
•Mild formula - will not cause damage to the target protein during antibody stripping and re-detection
•Odorless - no sulfur alcohol, no pungent odor
•Economically priced—lower price than competing stripping buffers

Product Details
Perform gel electrophoresis and use parallel duplex Using Western Blot analysis to detect new primary antibodies or antibody concentrations can be very time-consuming and expensive, and Restore Western Blot Antibody Stripping Buffer eliminates the waste associated with using chemiluminescent Western Blot substrates to detect Western blots. Restore Antibody Stripping Buffer completely and efficiently removes primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen, allowing the blot to be cleared and the antigen re-detected with still reliable results.

Chemiluminescent immunoblotting detection using Thermo Scientific SuperSignal Horseradish Peroxidase Substrate is currently the most common and sensitive method. Because these substrates do not precipitate and are permanently bound to the membrane surface, immunoblots detected by chemiluminescence can be stripped using this reagent, thereby removing affinity-bound primary and secondary antibodies. To achieve good results, the antibody stripping buffer needs to be strong enough to separate bound antibodies and gentle enough to retain the transferred target protein on the nitrocellulose or PVDF membrane. Restore Western Blot Antibody Stripping has these characteristics.

By stripping and re-detecting, we eliminate the need to run multiple gels to detect different targets, thereby wasting scarce or expensive samples. A membrane produced by one gel electrophoresis can be removed from the blot by using Restore Western Blot Antibody Stripping Buffer to remove the primary antibody. Blot stripping only takes 15 to 30 minutes, depending on the affinity of the primary antibody. After the blot is removed, a new primary antibody can be used to rebind and detect it. Additionally, adjusted antibody concentrations can be used to rebind the blot after stripping to further optimize experimental conditions if initial results are poor.

Applications
•Reusable nitrocellulose or PVDF membranes to detect different targets with different primary antibodies
•Blots can be re-tested, corrected or optimized Ineffective Antibody Concentrations in Initial Experiments

Protocol Summary
• Rinse the blot to remove the chemiluminescent substrate.
•Incubate the blot in Restore Western Blot Antibody Stripping Buffer for 5 to 15 minutes at 37°C (room temperature is sufficient for some antibodies).
• Remove the immunoblot and wash in wash buffer (TBS-T or PBS-T).
•Test whether the antibodies have been adequately removed.
•Conduct your next western blot experiment.