Ham's F-12K (Kaighn's) Medium is an improved version of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) medium was developed specifically for primary human hepatocytes and selected rat and chicken hepatocytes in a serum-reduced environment.

This Ham's F-12K (Kaighn's) medium is produced as follows:
Available in fullFormulation.

Usage of Ham's F-12K
Ham's F-12K (Kaighn's) medium contains many ingredients that are not found in traditional basal medium, such as putrescine and thymidine , hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. With the addition of these components, only minimal amounts of serum or well-defined components need to be supplemented in the culture medium for certain cell types. Ham's F-12K (Kaighn's) medium does not contain protein or growth factors, so it is often supplemented with growth factors and Fetal Bovine Serum (FBS). FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) medium uses a sodium bicarbonate buffer system (2.5 g/L) and therefore requires an environment of 5–10% CO₂ to maintain physiological pH.

cGMP production in two locations
To ensure the stability of the supply chain, Ham's F-12K (Kaighn's ) medium. Both locations comply with cGMP manufacturing requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.