Thermo Fisher Scientific Pierce His-Tag Protein Interaction Sedimentation Kit contains a method for capturing and purifying proteins that interact with His-tagged fusion proteins. required components.

His-Tagged Protein Interaction Sedimentation Kit Features:

•6xHis Sedimentation (Product No. 21277)—Purify protein interactors from any His-tagged fusion protein
•Complete kit—provides all components and detailed protocols for purified protein:protein interactions
•No special equipment required—uses common laboratory equipment and reagents (e.g., microcentrifuge)
•Convenience—Micro spin columns facilitate simple and efficient manipulation of agarose beads, including simple handling of multiple samples
•Flexibility— Instructions include protocols for use with bait and prey proteins expressed in a variety of sample types
•Less non-specific binding—Cobalt chelating resins are more effective than nickel resins Histidine-tagged fusion proteins are more specific and produce less non-specific binding
•Binding— 10 to 25 mg Histidine-tagged fusion protein/mL resin

< b>Applications:

• Discovery of a new protein from cell lysates: protein-protein interacting proteins
• Confirmation of putative interactions from cell lysates or previously purified proteins
•Protein extraction from in vitro transcription/translation lysates: Protein interaction information

You provide tagged fusion proteins as “baits” and express putative protein interaction targets (“prey” ) cells, the sedimentation kit provides additional: cell lysis buffer, micro spin column, tag-specific affinity resin (agarose beads) and optimized buffers and protocols. The sedimentation kit is designed to teach the method to first-time users and improve ease of use, convenience, and reproducibility for experienced researchers.

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