EZ-Link Amylamine-Biotin Features:

•Biotinylation—for use with avidin or streptavidin probes Labeled molecules and surfaces for detection or affinity purification methods of resins and resins
•Amine Activation—Primary amines can be cross-linked to proteins and material surfaces using EDC and other cross-linking agents
•< b>Medium length—The spacer arm (total length added to the target) is 18.9 Angstroms and represents the 6-atom extension of the native biotin valerate (and its conversion to the primary amine)

Pentylamine Bio Biotin is a compound formed by modifying the valeric acid side chain of biotin with pentane-1,5-diamine. The compounds contain a terminal primary amine (-NH2) that provides a functional handle for derivatization or coupling to proteins, surfaces, and other molecules. Carbodiimide (EDC) and NHS ester cross-linker chemistries are commonly used for covalent modifications involving amino-biotin molecules. The compounds may also be used as amine substitutes for native (carboxylate) biotin for assays and methods requiring biotin standards or blocking steps.

We manufacture biotin reagents to ensure the highest possible level of overall product integrity, consistency and performance for intended research applications.

Amino-biotin compounds can be coupled to functional groups on proteins and other molecules in a variety of ways. The most common method is to use Cross-links terminal primary amines to carboxyl groups. Carboxyl groups (-COOH) are present on aspartic acid or glutamic acid residues and on the carboxyl termini of polypeptides. When activated withEDC (Product No. 22980), the carboxylate reacts with the amino group (— NH2) group reacts to form an amide bond. Carboxylate molecules and surface materials can be prepared using EDC and Sulfo-NHS (Product No. 24510) Activated for subsequent reactions with primary amines (see NHS Ester Chemistry).

Applications:
•For colorimetric determination of factor XIII and cellular transglutaminase