Slide-A-Lyzer Mini Dialysis Cups are produced in a clean environment and automatically packaged after assembly to ensure they are free of contaminants and ready for use. These 3.5K MWCO dialysis cups are available in three sizes (0.1, 0.5 and 2 mL capacity) and are designed to allow easy addition and removal of samples using standard laboratory pipettes. The mini dialysis cup can efficiently and conveniently complete typical sample desalting or buffer exchange in 4–8 hours. Unlike other small sample separation devices, it requires no assembly equipment, no syringe adapters, and no laborious steps to process small sample volumes. Regenerated cellulose membranes are compatible with common chemicals and buffers.

Slide-A-Lyzer Mini Dialysis Cup (3.5K MWCO) Features:
• Three convenient sizes — 0.1, 0.5 and 2 Dialysis cups for mL samples fit standard 1.5 mL microcentrifuge tubes, 15 mL and 50 mL conical tubes
• Excellent sample recovery — low binding plastic and small membrane surface area Possible reduction of sample loss
• One-step protocol — pipette sample into mini cup and place into tube containing dialysis buffer; no laborious assembly, Unit Prep or Expensive Equipment
• 100% Leak Tested — Innovative design eliminates the "capillary siphoning" phenomenon that can occur in homemade equipment
• < strong>Save time — Desalt nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and binding

0.5 mL and 2 mL The mini dialysis cups are inserted into the supplied 15 mL and 50 mL conical tubes respectively and installed with the caps on. The tubes can be securely placed in standard laboratory racks or shakers; this feature saves space, minimizes the risk of leakage or contamination and eliminates the need for floats or labeled beakers of buffer. Low molecular weight contaminant removal, buffer exchange, and desalting can be accomplished in 4–8 hours using these mini dialysis cups, with efficiencies, rates, and recoveries very similar to conventional dialysis using larger dialysate volumes.

This 0.1 mL mini dialysis cup has a lid-fit design and can be inserted into a standard 1.5 mL microcentrifuge tube. Alternatively, up to 25 mini cups can be placed in the Slide-A-Lyzer Mini Dialysis Cup Float to dialyze multiple samples in a large beaker.

Using the 0.1 mL mini dialysis cup, 100 µL of pH 2.8 glycine buffer can be equilibrated with 1 liter of pH 9.4 bicarbonate buffer in 10 minutes. As with all dialysis applications, the dialysis rate observed for each sample using this type of dialysis cup depends on a variety of factors, including membrane surface area to sample volume ratio; temperature and agitation rate; molecular weight, shape, and degree of hydration of the molecules passing through the membrane and charge; as well as the pH, ionic strength, and solvent properties of the buffer used.