Pierce Coomassie (Bradford) protein detection kit is a ready-to-use, stable traditional Bradford detection reagent formula. Used to determine total protein concentration compared to protein standards. The kit includes Coomassie protein detection reagent and a series of albumin standard ampoules.

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Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of a widely used assay originally described by Bradford in 1976. When mixed with a protein solution, the color of the acid Coomassie dye reagent changes from brown to blue in a manner proportional to the amount of protein present in the sample. Protein determination is performed by comparison with the color response of protein detection standards typically prepared as known dilution series of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The simple program works on almost any volume scale, including test tubes, cuvettes, and microplates. Protein detection is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal chelators encountered in protein samples.

Coomassie Protein Assay Kit features include:
•Bradford Reagent—a stable, ready-to-use kit of the classic Bradford assay
• Colorimetric—Measured at 595 nm
using a standard spectrophotometer or microplate reader •Easy to use—Single reagent; no need to prepare working reagents
•Quick —Almost instant color development; add, mix, and read results
•Detection Range—Detect protein concentrations from 1 to 1500 μg/mL
•Flexible< /b>—Microplate and test tube solutions are available and suitable for several target working ranges

How Coomassie (Bradford) assay kits detect proteins
Based on test The color development in protein detection using Mas dye (Bradford) is related to the presence of certain basic amino acids (mainly arginine, lysine and histidine) in the protein. Van der Waals forces and hydrophobic interactions are also involved in the binding of proteins and dyes. The number of Coomassie dye bound to each protein molecule is roughly proportional to the number of positive charges on the protein. Free amino acids, peptides, and low molecular weight proteins do not produce color with Coomassie dye reagents. In general, a peptide or protein must have a mass of at least 3,000 daltons to be detected with this reagent.

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