Thermo Scientific Pierce Protein A/G Plus Agarose is an ultra-high capacity Protein A/G beaded agarose resin , suitable for various antibody affinity purification methods.

Protein A/G Plus Agarose Features:

•Protein A/G – immobilized recombinant fusion of the antibody binding domains of Protein A and Protein G Protein enables polyclonal IgG purification of virtually any mammalian species
• Agarose Resin – The support is cross-linked 6% beaded agarose (CL-6B), which is a protein affinity The most commonly used resin for and purification methods
• Inert and Stable – Superior production method immobilizes Protein A/G via uncharged leach-resistant covalent bonds, resulting in lower non-specific binding and Able to be used multiple times without loss of yield
• High Capacity – The “Plus” version of Pierce Protein A/G Agarose contains a dense loading of immobilized Protein A/G, providing greater than Binding Capacity of 50 mg Human IgG/mL Resin

Pierce Protein A/G Plus Agarose contains high-density covalently immobilized to high-quality cross-linked 6% beaded agarose (CL-6B) for purification. Protein A/G recombinant fusion protein. Because Protein A/G combines the immunoglobulin binding domains of Protein A and Protein G, this resin is effective for affinity purification of IgG antibodies from a variety of mammalian host species.

Protein A/G is a genetically engineered protein that combines the IgG binding domains of protein A and protein G. The fusion protein was expressed in E. coli. Protein A/G contains four Fc-binding domains of Protein A and two Fc-binding domains of Protein G, with a final molecular weight of 50,460 daltons (40 to 45 kDa measured by SDS-PAGE). Protein A/G is less pH dependent than Protein A alone, but shares the additive properties of Protein A and G. Protein A/G binds to all human IgG subclasses and is therefore ideal for purification of polyclonal or monoclonal IgG antibodies of unidentified subclasses.

Pierce Protein A/G Plus Agarose is prepared using Thermo Scientific AminoLink coupling chemistry, which offers several advantages over traditional ligand immobilization methods. After AminoLink immobilization, the sugar monomers of the agarose beads bind to the native lysine residues on protein A through simple amide bonds. Unlike typical cyanogen bromide (CNBr) immobilization methods, the AminoLink method does not introduce any new chemical groups, thereby avoiding possible non-specific binding and forming a stable binding that is essentially irreversible. The resulting high binding capacity resin can still retain the binding function of immobilized protein A/G after multiple rounds of antibody purification.

Properties of Cross-Linked 6% Beaded Agarose (CL-6B):
•Supports pH stability: 2 to 14 (short-term); 3 to 13 (Long term)
• Average particle size: 45 to 165 microns
• Exclusion limit: 10,000 to 4,000,000 daltons
• Maximum volume flow rate: approximately 1 mL/min (for 1 cm diameter column)< br>•Maximum linear velocity: 30 cm/h
•Maximum pressure: less than 25psi (1.5 bar); defined as the maximum pressure drop across the column that the resin can withstand (Note: The indicated gauge pressure of the LC unit may Measures the total system pressure, not the pressure drop across the column)

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