ReadyProbes® Cell Viability Imaging Kit (blue/red) is a ready-to-use assay kit that is fast and Easily measure cell viability. Simply add 2 drops each of room temperature stable NucBlue® Live Reagent (Hoechst 33342) and propidium iodide to 1 mL of cell growth medium and determine cell viability by counting total cells and dead cells. NucBlue® Live reagent stains the nuclei of all cells and can be detected using standard DAPI filters. Propidium iodide stains only the nuclei of cells with compromised membrane integrity and can be detected using the standard TRITC/RFP (orange) filter set. This kit is suitable for fluorescence microscopy, fluorescence microplate readers and flow cytometry analysis.

NucBlue® Live Reagent: Stains nuclei of all cells; detects using standard DAPI filters (maximum excitation/emission wavelength: 360/460 nm)
Propidium iodide: Stains only the nuclei of dead cells with damaged cell membranes; detect using standard TRITC/RFP (orange) filter set (maximum excitation/emission wavelength: 535/617 nm )
See other ReadyProbes® cell staining reagents
Learn more about other cell viability assay kits

Usage recommendations
• NucBlue® Live Reagent and propidium iodide can be added directly to cells in complete growth medium or in compatible buffers.
• In most cases, 2 drops/mL and an incubation time of 5 to 30 minutes will stain nuclei brightly; however, optimization may be required for some cell types, conditions, and applications. In this case, simply add more or less droplets until the best stain intensity is achieved.