Invitrogen Power Blotter 1-Step Transfer Buffer is designed for use with the Power Blotter System to transfer proteins from polyacrylamide gels ( SDS-PAGE) for rapid semi-dry transfer to nitrocellulose or PVDF membranes. This transfer buffer is compatible with commonly used detection methods such as staining, chemiluminescence or fluorescence detection.

Power Blotter 1-Step Transfer Buffer Features:
•Fast—When used with a compatible fast semi-dry transfer system, its high ionic strength formula can be used in 5 Complete protein transfer in 12 minutes
•Optimized—Designed for seamless and reliable performance with the Power Blotter System
•Compatible— Compatible with other semi-dry transfer equipment equipped with a suitable high-current power supply
•Economical—no special or expensive consumables required with other fast semi-dry transfer equipment

Learn More information about semi-dry transfer equipment: Power Blotter System

When used with the Power Blotter System, this buffer can be clotted in 5 to 12 minutes. High-efficiency protein transfer from gel to membrane, with efficiency comparable to traditional Western blotting technology. Fast blotting methods require the use of a high-current power supply (such as the Power Blotter System) and an optimized high-ionic strength transfer buffer (such as the Power Blotter 1-Step Transfer Buffer).

Excellent transfer efficiency can be obtained in less time than traditional methods by increasing the current. The current is kept constant (∼22–23 mA/cm²) according to the surface area of the transfer membrane group, and the voltage is no higher than 25 V.

Power Blotter 1-Step Transfer Buffer is compatible with other protein semi-dry transfer devices when paired with an appropriate high-current power supply. These devices provide a constant high current (1.3 to 5.0 A) to rapidly transfer proteins through the high ionic strength conditions provided by the transfer buffer.

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