When combined with a ViewRNA Tissue probe set (purchased separately), the ViewRNA Tissue Core Kit and ViewRNA Modules enable the colorimetric detection of mRNA targets in tissue sections using the Fast Red, Fast Blue, and DAB substrates.

ViewRNA Tissue assays are RNA in situ hybridization (ISH) assays for the reliable detection of one or two mRNA targets within tissue sections. Visualize any gene in any tissue with single-copy sensitivity and no radioactivity. And in case your needs are not met by one of our 6500 catalog probe sets, we have decades of bioinformatics expertise and will design custom probe sets to any mRNA target of interest, free of charge.

With ViewRNA Tissue assays, you can achieve:
• Robust, single mRNA molecule detection in tissue sections
• Accuracy and specificity inherent to branched DNA amplification technologies
• Simultaneous detection of two RNA targets

The ViewRNA Tissue Assay is compatible with formalin-fixed, paraffin-embedded (FFPE) and cryopreserved tissue. Whereas traditional ISH technologies often amplify both signal and background, ViewRNA assays use branched DNA (bDNA) technology to amplify target-specific signal and achieve superior signal-to-noise ratios.

To meet various experimental needs, the ViewRNA Tissue assay is provided in a variety of kits and modules. The ViewRNA Tissue Core Kit and ViewRNA Modules can be purchased separately in different combinations to mix and match and create a colorimetric ViewRNA assay for detection of up to two RNA targets. For added convenience, ViewRNA 1-Plex and 2-Plex Kits containing the ViewRNA Tissue Core Kit and ViewRNA Modules can also be purchased, then supplemented with the ViewRNA Tissue Core Kit and ViewRNA Modules as needed for additional assays or substrates. 

ViewRNA Tissue Core Kit
The ViewRNA Tissue Core Kit includes the ViewRNA Tissue Core Reagents (2-8°C reagents) and ViewRNA Tissue Wash Buffers (room temperature buffers) containing the reagents for in situ hybridization to RNA targets, but does not contain the specific target probes or colorimetric substrate for detection. The ViewRNA Tissue Core Kit can be combined with the ViewRNA Fast Red, Fast Blue, and DAB Modules which provide the substrates for RNA detection in ViewRNA colorimetric ISH tissue staining. The ViewRNA Tissue Core Reagents and ViewRNA Tissue Wash Buffers can also be purchased separately. Sufficient reagents are provided to stain 24 tissue sections.

ViewRNA Fast Red Module
The ViewRNA Fast Red Module includes the alkaline phosphatase (AP) substrate Fast Red to detect a single mRNA target with red colored precipitate for ViewRNA colorimetric ISH tissue staining. The Fast Red substrate is for use with ViewRNA Type 1 or Type 6 Probe Sets (ViewRNA probe sets are sold separately). Sufficient reagents are provided to stain 24 tissue sections.

ViewRNA Fast Blue Module
The ViewRNA Fast Blue Module includes the alkaline phosphatase (AP) substrate Fast Blue to detect a single mRNA target with blue colored precipitate for ViewRNA colorimetric ISH tissue staining. The Fast Blue substrate is for use with ViewRNA Type 6 or Type 1 Probe Sets (ViewRNA probe sets are sold separately). Sufficient reagents are provided to stain 24 tissue sections.

ViewRNA DAB Module
The ViewRNA DAB Module includes the horseradish peroxidase (HRP) substrate DAB to detect a single mRNA target with brown colored staining for ViewRNA colorimetric ISH tissue staining. The DAB substrate is for use with ViewRNA Type 4 Probe Sets (ViewRNA probe sets are sold separately). Sufficient reagents are provided to stain 24 sections.