Fluo-4 Calcium Imaging Kit is designed for specific detection of calcium flux through imaging applications. Upon binding of calcium, a large increase in fluorescence emission (>100-fold) can be detected. To minimize time to experimental results, the kit contains all the reagents needed to measure calcium flux in living cells.

Learn more about ion indicators, including calcium, potassium, pH, and membrane potential indicators›

Fluo-4 Calcium Imaging Kit features include:

•Fluorescence emitted by calcium-bound Fluo-4 stain (excitation/emission wavelength 494/506 nm) can be detected using standard FITC filters
•After binding calcium, the fluorescence intensity increases >100 times
•Buffer K_d for Ca²⁺ in solution: ∼335 nM
• Contains all necessary reagents, reducing time to experimental results
• Binds calcium Afterwards, the fluorescence increases and the wavelength slightly changes

Includes all necessary reagents
The Fluo-4 Calcium Imaging Kit contains all the necessary components needed to detect calcium flux through imaging applications point. In addition to Fluo-4 stain, the kit contains PowerLoad™ Concentrate, 10X Neuro Background Suppressor Solution, and probenecid.

Labeled calcium indicators (such as Fluo-4 stain) are molecules that increase fluorescence after binding calcium. Fluo-4 is an analogue of Fluo-3 with two key chemical differences that result in enhanced fluorescence excitation at a wavelength of 488 nm, resulting in a higher fluorescence signal level (fluorescence intensity increase after calcium binding >100 fold) . For ease of use, Fluo-4 stain is provided as a ready-to-use solution.

For easy cell loading, the Fluo-4 Calcium Imaging Kit includes PowerLoad™ Concentrate. Thanks to the unique properties of PowerLoad™ solution, it can be used in complete culture medium, thus reducing the negative effects of changing culture media or loading in serum-free media.

Baseline autofluorescence caused by components in the growth medium can be significantly reduced by adding the included Neuro Background Suppressor. This solution is specially formulated for use on neuronal cells and does not cause osmotic shock. Additionally, it has been successfully used in many different cell types to effectively suppress background fluorescence without affecting the specific cell fluorescence generated in the assay.

The Fluo-4 Calcium Imaging Kit also comes with the proprietary water-soluble probenecid (often used to inhibit cellular transport and therefore reduce baseline signal).