Thermo Scientific Pierce BS³ (Sulfo-DSS) is a bis(sulfosuccinimide Amine) suberate, a homobifunctional, water-soluble, non-cleavable and membrane-impermeable amine-amine cross-linker.

BS³ An amine-reactive N-hydroxysulfosuccinimide (NHS) at each end of the 8-carbon spacer arm ester. NHS esters react with primary amines at pH 7-9 to form stable amide bonds and release the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, often have several primary amines in the side chains of lysine (K) residues and at the N-terminus of each polypeptide, and these primary amines can serve as targets for NHS ester cross-linking reagents.

No-Weigh Microtube Processing:
Eight microtubes each contain 2 mg of cross-linker. Pierce the aluminum foil with a pipette tip and add DMSO or DMF to DSS or water buffer to BS³. Store microtube drain plugs in the aluminum foil bags provided. Used microtubes can be cut off from unused microtubes and discarded.

Features of BS³:

•Reactive groups:Sulfo -NHS Esters(both ends)
• Can react with the following groups:Amino groups (primary amines)
• Amine-reactive Sulfo-NHS Esters with Reacts rapidly with any primary amine containing molecule
•Water soluble; comparable to DSS
• Membrane impermeable for cell surface labeling
• High purity crystallization reagent can be used to generate high purity cross-linked conjugates

Because it contains hydrophilic sulfonyl groups, BS³ The cross-linker is soluble in water and many common buffers up to ∼100 mM, thus avoiding the use of organic solvents that may disrupt protein structure. The water-soluble analogue of BS³, DSS, is also suitable for applications requiring a less hydrophilic cross-linker (e.g. to produce intracellular cross-links). DSS and BS³ have essentially the same cross-linking activity for primary amines.

Features of BS³:
• Other names: Sulfo-DSS
• Molecular formula: C₁₆H₁₈N₂O₁₄S₂Na₂
• Molecular weight: 572.43
• Spacer arm length: 11.4Å (8 atoms)
• CAS number: 82436-77-9
• Reactive group: NHS ester, at pH 7.0–9.0 with primary Reacts with amines

Applications:
• Cross-linking of cell surface proteins prior to cell lysis and immunoprecipitation
• Recognition of receptor-ligands by chemical cross-linking Interactions
• “Fix” protein interactions to identify weak or transient protein interactions
• Identify nearby protein interactions
• Perform protein cross-linking to create bioconjugations in a single-step reaction Material
• Immobilize proteins to amine-coated surfaces

BS³ Specifications:
We manufacture BS< to higher specifications sup>3 to produce highly specific bioconjugates, ensuring the integrity of your data and providing you with a high degree of consistency. Every batch of BS³ is tested and meets the following minimum specifications:
•Purity: >93% (quantified by NMR, the higher standard for cross-linker purity)
>• Solubility: > 5.8 mg/mL (in DI water), clear solution without insoluble materials

Product Reference
Crosslinker Application Guide -- Search Newer references for this product

Related products
Pierce ™ Premium BS3
DSS (disuccinimide suberate)
BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberic acid )
BS(PEG)9 (PEGylated bis(sulfosuccinimide) Suberic acid)