Ca²⁺ Ionophore A-23187 is commonly used for fluorescence In-situ calibration of Ca²⁺ indicators, balancing intracellular and extracellular Ca²⁺ concentrations, and facilitating the entry of Mn²⁺ into cells to make intracellular dyes Fluorescence quenching. Although the endogenous fluorescence of A-23197 is very strong and cannot be used with calcium indicators such as fura-2, indo-1, and quin-2 that can be excited by ultraviolet light, it is suitable for use with fluo-4, fluo that can be excited by visible light. -3. Use together with indicators such as Calcium Green and rhod-2. A-23187 can also be used to balance intracellular and intracellular Mg²⁺ concentrations, making it suitable for calibrating Mg²⁺ indicators.

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