Thermo Scientific Pierce Sulfo-SANPAH is an amine-reactive N-hydroxysuccinimide (NHS) ester and photoactivated nitro group Heterobifunctional cross-linkers for phenyl azides. NHS esters react efficiently with primary amine groups (-NH2) in pH 7–9 buffers to form stable amide bonds. This reaction releases sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azide forms a nitrene group that initiates an addition reaction via a double bond, inserts CH and NH sites, or subsequently undergoes ring expansion with nucleophiles (e.g., primary amines) reaction. Sulfo-SANPAH is provided as a sodium salt and is used for cell surface protein cross-linking. It is charged and water-soluble and can be dissolved at a concentration of 10 mM.

Thermo Scientific No-Weigh products are cross-linking reagents provided in small pre-portioned packages. The pre-packaged form is ready-to-use to prevent the reagent from decreasing in reactivity and contamination over time. With this packaging format, one vial of fresh reagent can be used at a time, eliminating the operational hassle of weighing small quantities of reagents and reducing concerns about reagent stability.

Features of Sulfo-SANPAH:

•Reactive Groups: sulfo-NHS ester and Nitrophenyl azide
• Reacts with the following groups: Amino groups and any nucleophile
• Not cleavable
• N-Sulfosuccinimide-6-(4'-azido-2'-phenylnitroamino) caproic acid
• In Optimum photolysis occurs at 320-350 nm, minimizing radiation damage to biomolecules
• Water soluble; non-cleavable

Product Reference:
Cross-linking Agent Application Guide -- Search the latest references for this product