Thermo Scientific Pierce Anti-DYKDDDDK Magnetic Agarose is a product derived from in vitro protein expression systems, bacteria, yeast and mammalian cells A fast and convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins. The amino acid sequence DYKDDDDK (commonly known as FLAG) is recognized by a high-affinity rat monoclonal antibody (clone L5) covalently linked to magnet-embedded agarose core particles.

For protein purification, magnetic agarose is added to samples containing DYKDDDDK-tagged proteins, where the tag is located at the N or C terminus. The captured proteins are then magnetically separated from the supernatant, non-specific binding proteins are washed away, and the bound DYKDDDDK-tagged proteins are separated using an elution buffer. Use an instrument such as a magnetic stand or KingFisher Flex Magnetic Particle Processor to remove the magnetic agarose from the solution. Automated instruments are particularly useful for higher throughput purification and screening of purification conditions.

Features include:
Specificity — Unique base beads and highly specific antibodies minimize off-target binding (low non-specific binding)
High Purity — Optimized bind-wash-elute protocol enables high purity
High Yield — Special antibody coupling method enables high yield
Fast — The entire purification protocol typically takes less than 40 minutes
Economical — Purification protocols can be reused multiple times
Universal — Beads compatible with manual and automated workflows (e.g. KingFisher instruments)

Pierce Anti-DYKDDDDK Magnetic Agarose Properties:

Composition: Anti-DYKDDDDK antibody is covalently linked to a magnetic, highly cross-linked agarose carrier
Magnetization: Ferrimagnetic, low remanence
Bead size: 10–40 µm
Bead Concentration: 25% slurry in phosphate buffered saline, 0.01% Tween-20 detergent, 0.02% sodium azide, pH 7.2
Binding capacity:≥3.2 mg DYKDDDDK-tGFP-His protein (∼32 kDa)/mL precipitation beads