Thermo Scientific Pierce GalNAz (tetraacylated N-azidoacetyl galactosamine) is an azide label of sugars, providing a highly specific method for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.

Characteristics of azido-sugar:

•Bioorthogonality—azido groups are small, non-reactive, and not found in living systems medium; therefore azido-sugar compounds do not interfere with endogenous cellular pathways and displace their naturally occurring analogues
• Compatibility—Efficient use of phosphate under simple buffer conditions Compounds undergo reaction chemistry; no auxiliary reagents such as copper or reducing agents are required
• Chemical Selectivity—Azide and phosphine groups do not react with or interfere with components of biological samples Instead, they couple to each other efficiently
• Universal—The azide label can be used for detection, immobilization, coupling, or affinity purification, depending on which phosphine-activated compound it reacts with< br>
These sugars are azide derivatives of natural monosaccharides, and cells use post-translational modification biochemical pathways to glycosylate proteins. The azide functional group is small and does not react with endogenous molecules. When delivered to cells, these compounds are integrated by glycosylation events to effectively "tag" glycoproteins with azide groups. The azide group can then be used exclusively using alkyne activating reagents ("click" chemistry) or phosphine activating reagents (Staudinger connection) or occasionally Union.

When used in combination with phosphine-activated fluorescent dyes, biotin reagents, or other compounds, these azide-modified sugars facilitate the study of cellular pathways involving glycosylation.

There are several classes of glycoproteins grouped according to carbohydrate type and amino acid bond sites. N-linked glycosylation is a modification of asparagine, whereas O-linked glycosylation proceeds via hydroxyl groups of serine and threonine residues. Azide-modified sugars are metabolic substitutes for endogenous amino sugars. ManNAz is converted by cells into azidosialic acid derivatives forN-linked glycosylation of cell surface proteins. GlcNAz and GalNAz are mainly used to markO-linked glycosylation (O-GlcNAc andO-GalNAc).

Related products
GlcNAz (tetraacylated N-stack Nitroacetylglucosamine)
ManNAz (tetraacylated N-azidoacetyl Mannosamine)