• MS Compatible—The reagent is directly compatible with in-solution peptide digestion
• Sensitive—The procedure successfully enriches low-abundance proteins (low ng range)
> • Low background—minimal detergent residues in enriched samples
• Reduced antibody contamination—streptavidin compared to other IP methods Antibody biotinylation for protein enrichment reduces contamination in the eluate
• Robust—Procedures and reagents have been validated using multiple targets to ensure low-abundance proteins (ng Consistent enrichment of at least 2 peptides per protein

Thermo Scientific Pierce MS-Compatible Magnetic IP Kit (Protein A/G) uses high-quality Pierce Protein A/G magnetic beads to provide Provides broader antibody capture flexibility than using Protein A or G alone. The Thermo Scientific Pierce MS Compatible Magnetic IP Kit (Streptavidin) uses high-quality Pierce Streptavidin magnetic beads for use with biotinylated antibodies, which can be purchased commercially or through Labeling using IP with Pierce Antibody Biotinylation Kit. After the immunoprecipitation procedure, the target-enriched elution fraction can be used directly for in-solution trypsin digestion for LC-MS analysis without the need for gel purification, detergent removal, or desalting. Additionally, the reagents and procedures have been validated using manual and automated magnetic separations.

Applications
• Enrich low-abundance targets to improve MS sensitivity
• Identify target proteins not detectable by Western blot
• Perform by LC-MS analysis Quantitative determination of target protein
• IP-MS Antibody Screening