Thermo Scientific Pierce Streptavidin Magnetic Beads improve the efficiency of automated magnetic purification of biotinylated molecules.

Features of Streptavidin Magnetic Beads:

• High Performance—aggregation-free, pre-blocked superparamagnetic iron oxide particles for automated high-pass Provides exceptional consistency through volume handling and manual operation
• Stable cross-linking technology—Streptavidin is cured with anti-leak cross-linking technology
• High binding capacity—With high binding capacity, it can quickly and efficiently purify biomolecules in complex samples
• Low non-specific binding—Stable pre-blocked magnetic beads can Obtain high-purity products (such as antigens eluted with biotinylated antibodies in IP experiments), suitable for mass spectrometry and other downstream analytical applications
• Excellent performance—The binding capacity is unparalleled by other brands of magnets About three times that of bead products, the dosage for a single experiment is lower

Applications:
• Use biotinylated antibodies to immunoprecipitate antigens from multiple sources
• Immunoprecipitation of interacting complexes using biotinylated antibodies
• Capture protein interactors in pull-down experiments using biotinylated “bait” proteins
• Isolation of biotin tags from cell or tissue extracts DNA-protein complexes
• Capture single-stranded biotinylated DNA oligonucleotides
• Isolate biotinylated PCR products

Pierce streptavidin magnetic beads have been validated and optimized to It can be used on high-throughput magnetic platforms, such as Thermo Scientific KingFisher 96 and KingFisher Flex instruments. In addition, a suitable magnetic stand can be used to provide better performance for simple manual laboratory operations. This superparamagnetic iron oxide particle has better performance than other brands of magnetic beads (high binding capacity and low non-specific binding).

Pierce streptavidin magnetic beads use a recombinant streptavidin with a mass of 53kDa and an isoelectric point (pI) close to neutral. The protein is a tetramer with four biotin-binding sites. Unlike avidin, streptavidin does not contain sugar groups and therefore has very low non-specific binding. The high-affinity binding between streptavidin and biotin will only be destroyed under very harsh conditions, such as boiling in SDS-PAGE loading buffer or 8M guanidine·HCl at pH 1.5. . Therefore, typically the biotinylated bait protein will not be eluted at the same time as the capture protein in the interacting complex.

Related resources:
A review of automated magnetic separation of proteomes (including selection guide)

Related products:
Other Pierce magnetic beads