SuperBoost™ Tyramide Signal Amplification is the ideal solution for detecting multiplex fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC) and in situ The most sensitive method for low-abundance targets in hybridization (ISH) applications. SuperBoost kits combine the brightness of AlexaFluor™ dyes with the excellent signal amplification of poly-HRP-mediated tyramine labeling reactions, with sensitivity 10-200 times equivalent to standard methods. For superior research results, SuperBoost kits provide sharper definition of critical areas that imaging methods struggle to provide.

SuperBoost kits are easy to use and can be easily and flexibly adjusted according to standard ICC, IHC or FISH experimental protocols and are suitable for any type of cells or tissues. Cells labeled with the SuperBoost kit are compatible with any type of microscope, producing high-resolution images for multiplex analysis. This specific kit contains Biotin XX Tyramide with HRP-conjugated strepavidin. For detection and additional signal amplification, conjugated streptavidin is used to detect specifically deposited biotin XX.

SuperBoost kit features include:
• Ultra-high sensitivity detection of low-level or complex targets through fluorescence imaging technology
• Simple protocol and detection process using standard filters
• Suitable for high-resolution multiplex analysis images co-labeled with DAPI, secondary antibodies or other SuperBoost kits
• Compared to standard ICC/IHC/ISH experiments, the amount of primary antibody required is reduced by 10-100 times< br>
SuperBoost kit is based on the tyramine signal amplification system and utilizes the catalytic activity of horseradish peroxidase (HRP) to label target proteins or nucleic acid sequences in situ at high density. Ordinary ICC/IHC/ISH experiments using SuperBoost kits require 10-100 times less primary antibody than standard ICC/IHC/ISH experiments. SuperBoost kits provide specific signal intensity that is superior to background, making it easy to optimize protocols to detect specific signals in samples where high endogenous autofluorescence is observed.