Vybrant™ DyeCycle™ Green stain is a cell-permeable DNA dye that uses the 488 nm spectral line of the argon ion laser For cell cycle analysis, this laser is commonly used in all flow cytometers.

• Precise—accurate cell cycle analysis in live cells

• Safe—lower cytotoxicity in cell sorting and other live cell experiments

• Simple, Reliable staining protocols

ViewSelection guide for all products related to cell cycle analysis of fixed and living cells in flow cytometry .

Accurate

Successful cell cycle analysis requires dyes that are DNA-selective and stain cells in a uniform pattern with minimal fluorescence variability. Vybrant™ DyeCycle™ Green stain is ideal for analyzing DNA content in living cells because the stain is cell permeable and, upon binding to double-stranded DNA, emits a fluorescent signal proportional to the DNA mass (see figure).

Low Cytotoxicity

Unlike stains that require high concentrations or chemical structures that are cytotoxic, Vybrant™ DyeCycle™ Green stain exhibits relatively low The cytotoxicity can be sorted according to cell cycle stage.

Simple, reliable staining protocol

For cell analysis, prepare only flow cytometry tubes containing 1 mL of cells at 1 × 10⁶ cells/mL. Add 2 µL of Vybrant™ DyeCycle™ Green stain to each tube. The final stain concentration is 10 µM. Incubate for 30 minutes at 37°C in the dark. Analyze samples in a flow cytometer using 488 nm excitation and green emission wavelengths.