Thermo Scientific Pierce MA(PEG)12 is a methyl- and amine-terminated polyethylene glycol reagent that can be used in a variety of Surface modification and molecular PEGylation applications.

MA(PEG)12 Features:

• Fully characterized pegylation reagent with defined PEG chain length; molecules of discrete molecular weight ensuring consistent performance in protein modification applications< br>• PEG spacers offer unique advantages, including greater stability, lower tendency to accumulate, and lower immunogenicity
• Allow site-specific labeling of primary amines or carboxyl groups on proteins or surfaces or cross-linking
• Easy-to-follow instructions increase the likelihood of successful results

MA(PEG)n are a group of polyethylene glycol (PEG) spacers with methyl groups ( Abbreviation for compounds with -CH3) and amino (-NH2) groups. Unbranched, hydrophilic, discrete-length molecules have the methyl-PEGn-amine form, where the subscript "n" indicates 4, 8, 12, or 24 ethylene glycol units. The terminal primary amine of each compound provides a specific target for cross-linking and other coupling methods, allowing these compounds to be used as PEGylation reagents.

Applications of PEGylation:
• PEGylation of carboxylate or amine surfaces
• Addition to proteins, immunogens, pharmaceutical compounds and probes Inert substances
• Increase the solubility of proteins or peptides (reduce agglomeration) without affecting functionality
• Protect proteins from proteolysis

Methods of covalent attachment: < br>• Use EDC and Sulfo-NHS coupled to carboxyl
• Use DSS< /a>、BS(PEG)5/BS(PEG)9 or other NHS-ester reagent for cross-linking to primary amines
• Use Sulfo-SMCC or SM(PEG)n Reagents cross-linked to sulfhydryl groups
• via Reductive amination Attached to an oxidized carbohydrate group (aldehyde)

< b>Why is a protein or peptide pegylated?
Methyl-terminated PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, making it immune to proteolytic enzyme cleavage, increasing its half-life in biological applications, masking the immunogenic response it causes, and reducing its antigenicity or potential toxicity. Improves its solubility, reduces the potential for aggregation, and minimizes interference in in vitro and in vivo applications. Polyethylene glycol, also known as polyoxyethylene (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, soluble, and highly flexible.

Advantages of Discrete Length Polyethylene Glycol Compounds
These reagents are specially synthesized to produce homogeneous compounds with defined molecular weights characterized by discrete chain lengths that can be changed Better optimize and characterize surface protein modifications. Typical formulations of PEG compounds are heterogeneous mixtures containing a defined average molecular weight or approximate number of PEG subunits distributed in chain lengths.

Related products
MA(PEG) 4 methyl- PEG-Amine Compound
MA(PEG) 8 Methyl-PEG-Amine Compound
MA(PEG) 24 methyl-PEG-amine compound