Thermo Scientific Pierce CA(PEG) 24 is a PEGylated amino acid in the carboxyl-PEG-amine form, containing 24 polypeptides. Glycol units for a variety of surface and molecular PEGylation applications.

CA(PEG)n is an abbreviation for a group of compounds with a polyethylene glycol (PEG) spacer with carboxyl (-COOH) and amino (-NH2) termini. Unbranched, hydrophilic, Discrete length molecules have the carboxyl-PEGn-amine form, where the subscript "n" indicates 4, 8, 12, or 24 ethylene glycol units. The carboxyl and primary amine of each compound provide for cross-linking and other coupling methods. Specific targets, making these compounds useful as PEGylation reagents

Characteristics of CA(PEG)n compounds

• Well-characterized PEGylation reagents with well-defined PEG chain lengths. ; Molecules of discrete molecular weights ensuring consistent performance in protein modification applications
• Available as a range of ethylene glycol units (4, 8, 12 and 24 units) allowing modification procedures to be optimized to meet specific application needs , while retaining all the advantages associated with protein PEGylation
• PEG spacers offer unique advantages, including greater stability, lower propensity for aggregation, and lower immunogenicity
• Allow for protein PEGylation or primary amines or carboxyl groups on the surface for site-specific labeling or cross-linking
• Easy-to-follow instructions increase the likelihood of successful results

Applications of PEGylation
• PEGylated carboxyl or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve protein or peptide solubility without affecting functionality (Reduces accumulation)
• Protects proteins from proteolysis

Methods of covalent attachment
• Use EDC and Sulfo-NHS derived or cross-link carboxyl or primary amines
• Use DSS, BS(PEG)5/BS(PEG)9 Other NHS-ester reagents cross-link amino termini to primary amines
• Use Sulfo-SMCC or SM(PEG)n reagent cross-links the amino terminus to Thiol

Why PEGylate proteins or peptides?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, prevent proteolytic enzyme cleavage, increase its half-life in biological applications, mask it to avoid immunogenic reactions, reduce its antigenicity or potential toxicity, and increase its solubility. Reduce the potential for accumulation and minimize interference in in vitro and in vivo applications. Polyethylene glycol, also known as polyoxyethylene (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, soluble, and highly flexible.

Advantages of Discrete Length Polyethylene Glycol Compounds
These reagents are specially synthesized to produce homogeneous compounds with defined molecular weights characterized by discrete chain lengths that can be changed Better optimize and characterize surface protein modifications. Typical formulations of PEG compounds are heterogeneous mixtures containing a defined average molecular weight or approximate number of PEG subunits distributed in chain lengths.

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