Thermo Scientific Pierce Sulfo-SDAD (Sulfo-NHS-SS-Diaziridine) is activated by long-wavelength UV light. Validated photochemistry based on NHS esters and bisaziridine is combined with the coupling of amine-containing molecules with virtually any other functional group. A 13.5Å spacer arm containing a cleavable disulfide bond separates the two photoreactive groups.

Features of Sulfo-SDAD (Sulfo-NHS-SS-Diaziridine):

•Water solubility—increased solubility in aqueous solutions through sulfonic acid groups
•Heterobifunctional—NHS ester group reacts with primary amines at pH 7 to 9 to form covalent amides key; when activated with long-wavelength UV light (330-370 nm), Diaziridine (azidopentanoic acid) group can be associated with any amino acid side chain or peptide The backbone reacts efficiently
•Controlled—Use common laboratory UV lamps to activate two-step chemical cross-linking
•Easy to use—These cross-linkers are available in Photostable under typical laboratory lighting conditions, eliminating the need to perform experiments in the dark
•Better than aromatic azides—The bisaziridine photoreactive group remains stable under normal lighting conditions It has better photostability than the traditional photoreactive cross-linker phenyl azide group, and long-wave UV light can activate the bisaziridine group more efficiently

Succinimide-Bis Emineridine (SDA) reagents are a new class of cross-linkers that combine proven amine reactivity chemistry with an innovative, high-efficiency photochemistry based on bisazidine for coupling amine-containing molecules to virtually on any other functional group. SDA cross-linkers include six compounds with varying spacer arm lengths that are capable of cleaving cross-linked proteins in the presence or absence of charged groups for membrane permeability. Protein cross-linking is an important technique for understanding protein structure and stabilizing protein-protein interactions, and these SDA reagents expand the efficiency and range of interactions that can be explored with this method.

Typical bifunctional amine or sulfhydryl reactive cross-linkers require specific amino acid groups (such as lysine or cysteine) at the correct distance on both proteins to capture protein interactions. The SDA cross-linker allows specific labeling of one protein through the use of amine-reactive N-hydroxysuccinimide (NHS) esters and then through the use of bisazines attached to any amino acid side chain or peptide backbone of another protein. UV-activated cross-linking achieved with the pyridine moiety circumvents this limitation. Bisaziridine-based photocrosslinkers have better photostability than phenylazide-based photocrosslinkers and are easily activated using long-wavelength UV light (330-370 nm).

A wide range of bisethylene cross-linkers are available
NHS ester bisethylene derivatives (SDA, LC-SDA and SDAD) lack charged groups and Membrane permeable. This property makes it suitable for intracellular and intramembrane coupling. In contrast, Sulfo-SDA, Sulfo-LC-SDA, and Sulfo-SDAD contain negatively charged sulfate groups that increase their water solubility and reduce cell membrane permeability, thereby facilitating their extracellular use. Protein cross-linking. SDAD and Sulfo-SDAD also have disulfide bonds within the spacer that can be cleaved by reducing agents.

• Short (3.9Å) and long (12.5Å) spacer types
• Membrane permeable (NHS) and impermeable (Sulfo-NHS) types
• Non-cleavable and cleavable (disulfide spacer) types

Related products
SDAD (NHS-SS-Diaziridine)
Sulfo-SDA (Sulfo- NHS-Diaziridine)
Sulfo-LC-SDA (Sulfo-NHS-LC- Diaziridine)