pcDNA™3.3-TOPO™ TA Cloning™ Kit is designed for use in adherent cells as well as FreeStyle™ MAX CHO and FreeStyle™ MAX 293 achieves unusually high levels of transgene expression in suspension cell systems. The pcDNA3.3™-TOPO™ TA Cloning™ Kit comes with TOPO™ adapted plasmid vectors, all reagents for cloning, and One Shot™ TOP10 competent cells. The pcDNA™3.3-TOPO™ vector has the following advantages:

•TOPO™ is suitable for cloning PCR products with >85% efficiency
• Due to the presence of the native cytomegalovirus (CMV) immediate early promoter/ Enhancer sequence (672 bp) achieves high-level gene expression in a variety of mammalian cells
• Vector is suitable for large-scale protein expression
• Can produce native proteins without producing exogenous amino acids
< pcDNA™3.3-TOPO™ vectors are also included in the OptiCHO™ Antibody Expression System for cloning and expression of antibody light and heavy chains.