MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed to magnetically deplete lineage-committed mouse hematopoietic cells from mouse bone marrow. . It uses a cocktail of antibodies designed for the hematopoietic lineage and streptavidin-coated magnetic beads. Lineage-committed cells bind to the antibody and subsequently to the magnetic beads. When placed in a magnetic field, lineage-negative cells can be separated from lineage-committed cells by decantation.

MagniSort™ Mouse Hematopoietic Lineage Depleted Antibody Cocktail contains the following antibodies:
Anti-mouse CD2 Biotin
Anti-mouse CD3 Biotin
Anti-mouse CD5 Biotin
Anti- Mouse CD11b Biotin
Anti-mouse CD19 Biotin
Anti-mouse CD45R Biotin
Anti-mouse Ly-6G (Gr-1) Biotin
Anti-mouse TER-119 Biotin

After removal, anti-mouse CD34 antibody (clone number RAM34) can be used to stain to verify the enrichment of multipotent progenitor cells. Anti-mouse CD150 antibody (clone number mShad150) and anti-mouse CD48 antibody (clone number mShad150) can also be used to verify the enrichment of multipotent progenitor cells. Additional staining of clone number HM48-1) confirmed CD34 hematopoietic stem cell enrichment.

Reported Applications
Magnetic Cell Separation